课程简介
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课程大纲
课程章节
- CHAPTER ONE: 课程资料
- CHAPTER TWO: a brief history
- CHAPTER THREE: The molecular nature of genes
- CHAPTER FOUR: The transcription apparatus of prokaryotes
- CHAPTER FIVE: Fine control of prokaryotic transcription
- CHAPTER SIX: The transcription mechanism of eukaryotes
- CHAPTER SEVEN: Translation-protein synthesis
- CHAPTER EIGHT: Replication-DNA synthesis
- CHAPTER NINE: Molecular cloning methods
- CHAPTER TEN: Molecular tools for studying genes and gene activity
- Supplement
CHAPTER ONE: 课程资料
1.1 冷冻电镜虚拟仿真实验
1.2 电子教材
1.3 课程思政
CHAPTER TWO: a brief history
2.1 1. A brief history
CHAPTER THREE: The molecular nature of genes
3.1 2. The molecular nature of genes
CHAPTER FOUR: The transcription apparatus of prokaryotes
4.1 3. RNA pol and promoter in prokaryotes
4.2 4. Initiation and extension of transcription
4.3 5. Termination of transcription
CHAPTER FIVE: Fine control of prokaryotic transcription
5.1 6. The Lac operon
5.2 7. Fine control of lac operon
5.3 8. The trp operon
5.4 9. Fine control of trp operon
CHAPTER SIX: The transcription mechanism of eukaryotes
6.1 10. Eukaryotic RNA polymerases and promoters
6.2 11. Enhancer Silencer and Insulator
6.3 12. Eukaryotic transcription factors
6.4 24. Splicing
6.5 26. Capping and Tailing
CHAPTER SEVEN: Translation-protein synthesis
7.1 15. Preparation for translation
7.2 16. Initiation and elongation of translation
7.3 17. Termination of translation
CHAPTER EIGHT: Replication-DNA synthesis
8.1 18. Features of DNA replication
8.2 19. Enzymes in DNA replication
8.3 20. DNA replication process in Prokaryotes
8.4 21. DNA replication process in Eukaryote
8.5 22. DNA mutation and damage
8.6 23. DNA repair
8.7 24. Homologous and site-specific recombination
8.8 25. Transposon
CHAPTER NINE: Molecular cloning methods
9.1 26. Molecular cloning methods
9.2 27. Methods of expressing cloned genes
CHAPTER TEN: Molecular tools for studying genes and gene activity
10.1 28. Molecular hybridization
10.2 29. Real-time quantitative PCR
10.3 30. RNAi technology
10.4 31. CRISPR/cas9
10.5 32. Cryo-Electron Microscopy
Supplement
11.1 Phage display and yeast two-hybrid assay
11.2 GST pull-down and CO-IP
11.3 BiFC and filter-binding assay
11.4 EMSA, luciferase Assay and ChIP
11.5 DNA sequence
11.6 Lambda
